Occurrence, genetic characterization and antimicrobial resistance of extended spectrum β-lactamase producing Escherichia coli isolated from Sürk samples, a traditional turkish cheese
Journal of Food Measurement and Characterization, vol.10, no.3, pp.709-714, 2016 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 10 Issue: 3
- Publication Date: 2016
- Doi Number: 10.1007/s11694-016-9355-7
- Journal Name: Journal of Food Measurement and Characterization
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.709-714
- Keywords: CTX-M, E. coli, ESBL, Sürk cheese
- Hatay Mustafa Kemal University Affiliated: Yes
Abstract
This study was carried out to provide information regarding the presence of extended spectrum β-lactamase (ESBL) producing Escherichia coli isolated from Sürk cheese samples. For this purpose, ESBL-producing E. coli strains were isolated from 87 Sürk samples and isolates were screened for the common ESBL-types, presence of integrons and sul genes via PCR. All ESBL-producing E. coli strains were also allotted to phylogenetic groups and typed by PFGE. Twelve ESBL-producing E. coli (13.8 %) displayed eight distinct PFGE profiles and were exhibited resistance to tetracycline (41.7 %), trimethoprim-sulfamethoxazole (25 %), doxycycline (25 %) and chloramphenicol (16.7 %). Eleven isolates carried the blaCTX-M-15 gene (n = 7) and blaCTX-M-15 + blaTEM-1 genes (n = 4). Eleven isolates carried class1 and 2 integrons (intl1, 9 isolates and intl1 + intl2, two isolates) whereas four isolates carried sul1 (n = 2) and both sul1 + sul2 (n = 2) genes. The current study revealed a high rate of E. coli producing CTX-M-15 enzymes indicating a significant source for this organism in humans.