Multiplex PCR-based Molecular Characterization of Fecal-Derived Antibiotic Drug Resistant Escherichia coli Isolates Dışkı Kaynaklı Antibiyotik Dirençli Escherichia coli İzolatlarının Çoklu PCR Temelli Moleküler Karakterizasyonu
Adiyaman University Journal of Science, cilt.16, sa.1, ss.46-60, 2026 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 16 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.37094/adyujsci.1919076
- Dergi Adı: Adiyaman University Journal of Science
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.46-60
- Anahtar Kelimeler: ESBL, Escherichia coli, Integrons, fecal isolates, Multiplex PCR, Sulfonamide resistance
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
Antimicrobial resistance (AMR) in Escherichia coli poses a serious public health threat, particularly when resistant strains spread through contaminated food and water. This study characterized fecal-derived multidrug-resistant (MDR) E. coli isolates using multiplex polymerase chain reaction (PCR) to detect extended-spectrum β-lactamase (ESBL) gene variants (blaTEM, blaOXA, blaSHV, and blaCTX-M), sulfonamide resistance genes (sul1, sul2, and sul3), and integron-associated integrase genes (int1, int2, and int3). We analyzed 62 uspA-positive E. coli isolates. Results showed that 82.26% of isolates carried none of the targeted bla genes. The blaCTX-M and blaOXA genes were each present in 9.68% and 9.68% of isolates, respectively. Co-occurrence of bla and sul genes was observed in 8.06% of isolates. Co-occurrence of bla and int genes occurred in 14.52%. Only 8.06% of isolates carried at least one gene from all three categories, indicating potential MDR genotypes. These findings highlight the importance of molecular surveillance in tracking resistance genes outside clinical settings and support the need for integrated One Health strategies to combat AMR.