Serum and milk levels of antibodies to bovine viral diarrhoea virus, bovine herpesvirus-1 and -4, and circulation of different bovine herpesvirus-4 genotypes in dairy cattle with clinical mastitis
Journal of Veterinary Research (Poland), vol.67, no.1, pp.33-40, 2023 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 67 Issue: 1
- Publication Date: 2023
- Doi Number: 10.2478/jvetres-2023-0010
- Journal Name: Journal of Veterinary Research (Poland)
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.33-40
- Keywords: antibody, bovine herpesvirus-1, bovine herpesvirus-4, bovine viral diarrhoea virus, mastitis
- Open Archive Collection: AVESIS Open Access Collection
- Hatay Mustafa Kemal University Affiliated: Yes
Abstract
Introduction: Bovine viral diarrhoea virus (BVDV) and bovine herpesvirus (BoHV)-1 and -4 are important causes of respiratory diseases and reproductive disorders of dairy cattle worldwide. Material and Methods: Investigation of BVDV and BoHV-1 and -4 antibody levels in the serum and milk of dairy cattle in a group with clinical mastitis and a healthy group was undertaken using an indirect ELISA, and identification of the BoHV-4 genotypes in clinical mastitis cases was attempted by PCR and sequencing. Results: Antibodies specific to BVDV, BoHV-1 and BoHV-4 were detected in the serum and milk of all dairy cattle with clinical mastitis. The cut-off values for BVDV and BoHV-1 in the sera and milk were extremely high in both healthy and mastitic animals. However, BoHV-4 antibodies were detected only in the clinically mastitic cattle, and BoHV-4 levels were higher in milk than in sera among these animals. Genotypes I and II of BoHV-4 were detected in the milk samples of four seropositive cows with clinical mastitis from the same herd. Conclusion: The results of this investigation demonstrate that clinical mastitis cases in the same herd may have aetiology in different BoHV-4 genotypes.