Dentin tubule penetration of a calcium silicate-based sealer after removal of calcium hydroxide or N-acetylcysteine medicaments: an in vitro study
BMC ORAL HEALTH, cilt.26, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1186/s12903-026-08809-2
- Dergi Adı: BMC ORAL HEALTH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CINAHL, EMBASE, MEDLINE, Directory of Open Access Journals, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
Background This study aimed to evaluate, using confocal laser scanning microscopy (CLSM), the dentin tubule penetration of a calcium silicate-based root canal sealer after removal of calcium hydroxide (Ca(OH)(2)) or N-acetylcysteine (NAC) intracanal medicaments with needle irrigation or EDDY sonic activation. Methods Root canals of 128 extracted single-rooted mandibular premolars were prepared with ProTaper Next. Canals received Ca(OH)(2)(Kalsin) or (NAC) for 7 days. Medicaments were removed using conventional needle irrigation or EDDY sonic activation. Specimens were obturated with a calcium silicate-based sealer Bioserra using the single-cone technique. CLSM quantified residual medicament and sealer penetration into dentin tubules. Statistical analyses were performed using the Mann-Whitney U and Kruskal-Wallis tests, with the significance level set atp < 0.05. Results Neither the type of intracanal medicament nor the activation technique significantly affected the residual medicament amount (p > 0.05). However, the type of medicament significantly influenced sealer penetration, with NAC showing greater dentin tubule penetration than Ca(OH)(2) (p < 0.05). The activation technique did not significantly influence sealer penetration (p > 0.05). Sealer penetration was highest in the coronal third and lowest in the apical third (p < 0.05). Conclusion Within the limitations of this in vitro study, NAC enhanced the dentin tubule penetration of a calcium silicate-based sealer compared with Ca(OH)(2). The activation technique did not affect sealer penetration or residual medicament.