Bioactivity assessment and LC-MS/MS-based phenolic characterization of <i>Gypsophila pilosa</i>
SCIENTIFIC REPORTS, cilt.16, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 16 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1038/s41598-026-54804-6
- Dergi Adı: SCIENTIFIC REPORTS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE, Directory of Open Access Journals, Zoological Record, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
In this study, the antioxidant, anticholinesterase, antiproliferative activities, and phenolic compound profile of ethanol extracts of Gypsophila pilosa were comprehensively evaluated. Plant samples were collected from & Scedil;anl & imath;urfa province, T & uuml;rkiye, and after shade-drying under laboratory conditions, they were extracted with ethanol using a Soxhlet apparatus at 50 degrees C. The antioxidant capacity results revealed that total antioxidant status was 3.528 +/- 0.085 mmol/L, total oxidant status (TOS) was 10.248 +/- 0.072 & micro;mol/L, and oxidative stress index was 0.291 +/- 0.009. In addition, DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) were determined as 92.317 +/- 2.336 mg TE/g and 128.016 +/- 2.480 mg TE/g, respectively, indicating a strong antioxidant potential. In anticholinesterase assays, the extract exhibited inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with IC50 values of 160.163 +/- 2.155 & micro;g/mL and 223.903 +/- 1.049 & micro;g/mL, respectively. The antiproliferative activity was evaluated on A549 and MCF-7 cancer cell lines, as well as the HaCaT normal cell line. The extract demonstrated a dose-dependent reduction in cell viability in cancer cell lines, while comparatively lower cytotoxic effects were observed in normal cells at lower concentrations, suggesting a degree of selective activity. LC-MS/MS analysis revealed the presence of several bioactive phenolic compounds, including catechin hydrate, caffeic acid, gallic acid, and kaempferol, which are known for their antioxidant and cytotoxic properties. These compounds may contribute to the observed biological activities through overlapping and complementary mechanisms. In conclusion, G. pilosa exhibits notable antioxidant, enzyme inhibitory, and antiproliferative activities, which may be associated with its phenolic composition. However, further studies are required to clarify its selectivity and underlying mechanisms before potential pharmaceutical applications can be considered.