Serum matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-9/ neutrophil gelatinase-associated lipocalin complex levels in patients with early-stage diabetic nephropathy

Cakirca G., TURGUT F. H.

Iranian Journal of Kidney Diseases, cilt.12, sa.5, ss.299-304, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 5
  • Basım Tarihi: 2018
  • Dergi Adı: Iranian Journal of Kidney Diseases
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.299-304
  • Anahtar Kelimeler: Diabetic nephropathy, Extracellular matrix, Matrix metalloproteinase-9, MMP-9/NGAL complex, Tissue inhibitor-1
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Introduction. This study was aimed to investigate the efficacy of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), and the neutrophil gelatinase-associated lipocalin (NGAL)-bound form of MMP-9 (MMP-9/NGAL complex) markers in the determination of early nephropathy in patients with type 2 diabetes mellitus. Materials and Methods. Twenty-five type 2 diabetic patients with normoalbuminuria, 27 type 2 diabetic patients with microalbuminuria, and 23 healthy controls were recruited. Serum levels of MMP-9, TIMP-1, and MMP9/NGAL complex were measured in all participants. Results. The MMP-9 level and MMP-9/TIMP-1 ratio were higher in patients with microalbuminuria when compared to the controls, while TIMP-1 level was lower (P =.005, P =.006, and P =.02, respectively). The MMP-9 level and MMP-9/TIMP-1 ratio were significantly higher in the patients with normoalbuminuria when compared to the controls (P =.005 and P =.02, respectively). In the normoalbuminuric patients, MMP-9 levels were negatively correlated with estimated GFR (r =-0.553, P =.008) and positively correlated with glucose (r = 0.449, P =.04), creatinine (r = 0.454, P =.03), and MMP9/NGAL complex (r = 0.575, P =.005). In the microalbuminuric patients, MMP-9 levels were positively correlated with total cholesterol (r = 0.430, P =.03), and MMP9/NGAL complex (r = 0.650, P =.001). Conclusions. The levels of MMP-9, TIMP-1, and MMP9/NGAL complex were similar in microalbuminuric and normoalbuminuric patients with type 2 diabetes mellitus. However, there was a significant MMP-9/TIMP-1 imbalance in both groups which may reflect impaired extracellular matrix homeostasis.