The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion
Turkish Journal of Medical Sciences, vol.44, no.4, pp.576-581, 2014 (SCI-Expanded, Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 44 Issue: 4
- Publication Date: 2014
- Doi Number: 10.3906/sag-1307-100
- Journal Name: Turkish Journal of Medical Sciences
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Page Numbers: pp.576-581
- Keywords: BMP6, GDF-15, Hepcidin, HJV, Renal ischemia/reperfusion
- Open Archive Collection: AVESIS Open Access Collection
- Hatay Mustafa Kemal University Affiliated: Yes
Abstract
Background/aim: To determine the roles of hepcidin and its related genes in a renal ischemia/reperfusion model. Materials and methods: A total of 20 Wistar albino rats were equally divided into 2 groups: Group I was the control group and Group II was the ischemia and reperfusion (I/R) group (60 min of ischemia + 48 h of reperfusion). I/R was performed on the left kidneys of these rats and then the I/R-treated kidneys were removed. Te levels of serum biochemical markers were evaluated after renal I/R. Te expression levels of hepcidin-linked genes [growth differentiation factor 15 (GDF-15), bone morphogenetic protein 6 (BMP6), and hemojuvelin (HJV)] were also measured by RT-PCR technique. In addition, the tissues were evaluated histopathologically. Results: No significant association was found between renal dysfunction and I/R when compared to biochemical parameters (P > 0.05). However, differences in platelet values were statistically significant (P < 0.05). Expression levels of GDF-15, BMP6, and HJV genes increased, but this increase was not statistically significant. In addition, histopathological evaluation was performed using hematoxylin and eosin stain. This showed a significant relationship between the control group and I/R group for ischemic and nonischemic kidney scoring. Conclusion: Hepcidin and BMP6, HJV, and GDF-15 should be taken into account when investigating the process of I/R. © TÜBİTAK.