Akademik Veri Yönetim Sistemi
VIRAL HEPATIT DERGISI-VIRAL HEPATITIS JOURNAL, cilt.30, sa.2, ss.30-35, 2024 (ESCI, Scopus)
Objectives: This study aimed to investigate the seroprevalence of hepatitis E virus (HEV) and chronic HEV infection in patients with confirmed human immunodeficiency virus (HIV)-1 infection. Materials and Methods: This prospective single-center study included a sample of 101 patients aged 18-84 years who were admitted to Hatay Mustafa Kemal University Hospital between May 2022 and December 2022 with a confirmed diagnosis of HIV-1 infection. From the blood samples collected for the study, anti-HEV immunoglobulin M (IgM) [HEV IgM enzyme-linked immunosorbent assay (ELISA), DiaPro, Italy] and anti-HEV immunoglobulin G (IgG) (HEV IgG ELISA, DiaPro, Italy) were evaluated using the microplate ELISA method. Ribonucleic acid (RNA) was extracted from plasma samples using a Qiagen virus kit (Qiagen EZ1 automated system, Germany). HEV-RNA was tested using an in-house reverse transcription polymerase chain reaction for all samples included in the study. In addition, HIV-RNA, CD4+T cell count, hepatitis B surface antigen (HbsAg), anti-hepatitis C virus (HCV), antihepatitis A virus (HAV) IgG, aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin (TBIL), and venereal disease research laboratory (VDRL) parameters were obtained from the hospital automation system. Results: Anti-HEV IgG seropositivity was 3.96% (n=4). HEV-RNA was analyzed from all samples, but no HEV-RNA positivity was detected. There was no significant difference between the antiHEV IgG (+) and anti-HEV IgG (-) group in terms of HIV-RNA level, CD4+T cell count, ALT, AST, alkaline phosphatase, gamma-glutamyl transpeptidase, and TBIL levels. Anti-HAV IgG seropositivity was observed in 89.1% (n=90) of the patients included in our study, HbsAg seropositivity was observed in 1.0% (n=1), and HCV seropositivity was not observed. VDRL seropositivity was observed in 11.9% (n=12) of the patients. Conclusion: Although our findings showed that HEV seroprevalence was not high in HIV-positive patients and did not develop into chronic infection, we believe that multicenter studies with a larger number of patients are needed to investigate the relationship between HIV and HEV in our country.