Akademik Veri Yönetim Sistemi
Biochemical Systematics and Ecology, cilt.34, sa.2, ss.119-126, 2006 (SCI-Expanded)
There are difficulties in the morphological classification of two populations of Hedysarum pogonocarpum Boiss. in Turkey. Isozyme electrophoresis was employed to examine the genetic structure relationships of these two populations of H. pogonocarpum. Alcohol dehydrogenase, malate dehydrogenase and phosphoglycomutase isozymes were analysed by horizontal starch gel electrophoresis among the progeny grown from seeds collected from the Malatya and Kahramanmaras regions where it is the natural range of H. pogonocarpum. Of the four loci identified, ADH, MDH-1, MDH-2, PGM-2, only MDH-2 was monomorphic in both populations. The proportion of polymorphic loci in both populations of H. pogonocarpum was thus 75%, and the average allele number at each locus was 1.8. ADH isoenzymes in both populations were in the Hardy-Weinberg equilibrium, whereas for the MDH-1 locus there was more heterozygosity (P < 0.001) than expected. While the Kahramanmaras population was found to be in the Hardy-Weinberg equilibrium at the PGM-2 locus, the Malatya population displayed considerable deficiency (P < 0.01) in heterozygosity. About 10% of the total genetic variation was due to the interpopulational differentiation. The level of gene flow was found to be 2.05 per generation. Nei's genetic distance coefficient (0.112) and genetic identity (0.888) propose that two populations analysed could be accepted as subspecies of H. pogonocarpum. © 2005 Elsevier Ltd. All rights reserved.