A histological analysis of regeneration in watermelon

Yalcin-Mendi N., Ipek M., Kacan H., ÇÜRÜK S., Sari N., Cetiner S., ...Daha Fazla

Journal of Plant Biochemistry and Biotechnology, cilt.12, sa.2, ss.147-150, 2003 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1007/bf03263176
  • Dergi Adı: Journal of Plant Biochemistry and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-150
  • Anahtar Kelimeler: Growth regulator, Histology, Regeneration, Watermelon
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of watermelon [Citrullus lanatus (Thunb) Matsum & Nakai] cv Crimson Sweet were cultured on MS medium containing combinations of benzyl adenine (BA) (0, 5, 10, 20 μM) and indole-3-acetic acid (IAA) (0, 0.5, 5 μM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained from the media containing 10 μM BA + 0.5 μM IAA and 20 μM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12days in culture which later developed into buds.