Flow cytometric analysis of the effects of methotrexate and vepesid on the HEp-2 cell cycle


Duran N., Allahverdiyev A. M., ÇETİNER S.

Turkish Journal of Medical Sciences, vol.31, no.3, pp.187-192, 2001 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 3
  • Publication Date: 2001
  • Journal Name: Turkish Journal of Medical Sciences
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.187-192
  • Keywords: Cell cycle, Flow cytometer, HEp-2 cell, Methotrexate, Vepesid
  • Hatay Mustafa Kemal University Affiliated: No

Abstract

Objective: To determine the mechanism of action of Methotrexate and Vepesid on the HEp-2 cells isolated from human laryngeal cancer cells morphologically and flow cytometrically (G1, G2, S and PI). Materials and Methods: The HEp-2 continuous cell line was used. Cultivation of the cells was realized in EMEM medium with 10% fetal bovine serum at an atmosphere of 37°C with 5% CO 2. Six different concentrations of Vepesid and Methotrexate were prepared by diluting with deionized water (5 μg/ml, 50 mg/ml, 500 μg/ml). The morphological and cell cycle parameters of HEp-2 cells were determined by inverted microscope and flow cytometer respectively. Results: In the morphological examination. Vepesid was found to have a more significant cytopathologic effect on the cells than Methotrexate, whereas in the flow cytometric examination, it was found that whilst Methotrexate stopped the cells at the S and G 2 phases, Vepesid did that only at the G 1 phase. Conclusion: Both the flow cytometric and cell morphological analysis showed Vepesid to be more effective than Methotrexate on HEp-2 cells. Results of studies conducted show the mechanism of action of these drugs to be dependent on the origin of the cell and on the drug type.