POPULATION STRUCTURE ANALYSIS AND MOLECULAR CHARACTERIZATION OF DUCKWEED GENOTYPES USING iPBS AND ISSR MARKERS

COŞKUN Ö. F., AYDIN D.

Bangladesh Journal of Botany, cilt.53, sa.4, ss.871-880, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 4
  • Basım Tarihi: 2024
  • Doi Numarası: 10.3329/bjb.v53i4.77265
  • Dergi Adı: Bangladesh Journal of Botany
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.871-880
  • Anahtar Kelimeler: Duckweed, Genetic characterization, IPBS, ISSR, Lemna, Spirodela
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Duckweed belongs to subfamily Lemnoideae, considered as potential minor vegetable for human consumption. Genetic diversity and molecular characterization of 67 duckweed genotypes grown in different ecosystems were determined using ISSR (Inter Simple Sequence Repeats) and iPBS (Inter-Primer Binding Site) markers. ISSR and iPBS methods identified 100% polymorphism, with genetic similarity coefficients between Spirodela and Lemna species ranging from 0.17 to 0.56. Structure analysis conducted according to K=6 calculation revealed that L. minor had the most homogeneous and diverse populations. Duckweed genotypes and species displayed a wide range of genetic diversity. Using various marker techniques, the genetic structure of duckweed was determined and characterized it as an alternative, sustainable, and cheap source of protein, vegetables, and biofuel. The results obtained from this study will be useful for understanding molecular mechanisms in future genetic improvements of duckweed genotypes and species.