Akademik Veri Yönetim Sistemi
Medycyna Weterynaryjna, cilt.76, sa.1, ss.29-33, 2020 (SCI-Expanded)
The aim of the present study is to examine tbe effects of gentamlcln sulphate on spontaneous, oxytocin and PGF2a induced in vitro contractions of myometrium isolated from pregnant cows. Myometrial strips were obtained from healthy pregnant cows and suspended in a covered organ bath filled with Krebs solution at 37°C (pH 7.4) continuously bubbled with 95% oxygen and 5% carbon dioxide: Isometric contractions were recorded using an isometric force displacement transducer. After the stabilization of spontaneous contractile activity during a 90-minute equilibration period, contractions were recorded for 20 minutes (control). Gentamlcln sulphate was then added to the tissue bath cumulatively and the responses were recorded every 20-minutes for each consecutive dose of gentamlcln. In agonist-induced contractions, oxytocin or PGF2a was added to the tissue bath at the end of the equilibration period and the same protocol was followed to investigate the effects gentamlcln on these agonist-induced contractions. Gentamlcln decreased the frequency and inhibited the amplitude of the spontaneous contractions in a dose dependent manner (p < 0.05). The mean frequency and amplitude of oxytocln-induced contractions was significantly inhibited by the application of gentamicin (p < 0.05). Gentamlcln also inhibited the contractions induced by PGF2a in a dose dependent manner (p < 0.05). This study showed gentamicin inhibited, depending on the dosage, oxytocin and PGF2a induced contractions of myometrium isolated from pregnant cows. Upon clinically examining the findings obtained by the study, gentamicin can be used as an antibacterial in septic abort and chorioamniotis in order to prevent premature birth, abortion and early uterus contractions. Further studies are necessary to test whether the same effect will take place in vivo and to examine the effects of long-term use of gentamicin on offsprings.