Akademik Veri Yönetim Sistemi
Acta Medica Alanya, cilt.4, sa.1, ss.21-28, 2020 (Hakemli Dergi)
Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways. Materials and Methods: The MTT 3- (4,5-Dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide method was used to determine the appropriate doses of hydrogen peroxide ${H_2}{O_2}$ and M. communis L.’s essential oil in BEAS-2B cells. Oxidative stress formation was determined by measuring malondialdehyde (MDA) level. The cells were divided into three groups: the group exposed to oxidative stress (group with H2O2), the treatment group (${H_2}{O_2}$+ M. communis L.’s essential oil) and the control group. MDA levels were measured in all three groups and expression levels ofCaspase 3, Caspase 8, Caspase 9 and p21 genes were determined by RT-PCR method in order to detect apoptotic effects. Results: According to MTT test results, the appropriate doses were 40 μM for ${H_2}{O_2}$and 15.625 μg/ml for M. communis L.’s essential oil. MDA levels were significantly increased in the group treated with 40 μM H2O2 when compared with the healthy cell group (p=0.0005). In the group treated with essential oil of M. communis L., MDA level was found similar to the control group (p>0.05). Expression levels of Caspase 3, Caspase 8 and p21 genes were significantly increased in cells where ${H_2}{O_2}$ was adminisrated at 40 μM concentrations compared to healthy cell group (p=0.001, p=0.017 and p=0.0003, respectively). However, Caspase 9 gene expression level did not change significantly (p=0.8). Compared to the group in which the oxidative stress model was established, it was found that Caspase 3 gene expression level decreased significantly in the cells treated with M. communis L.’s oil (p=0.00007). Conclusion: In our study, it was shown that the essential oil of M. communis L. strongly decreased MDA levels and also had the potential to be a therapeutic agent due to its apoptotic inhibiting effect. M. communis L. has a strong antioxidant effect and is thought to be effective in stopping apoptosis caused by oxidative stress.