Preliminary characterization of race-specific elicitors from Peronospora parasitica and their ability to elicit phenolic accumulation in Arabidopsis

SOYLU S., SOYLU E. M.

Phytoparasitica, vol.31, no.4, pp.381-392, 2003 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 4
  • Publication Date: 2003
  • Doi Number: 10.1007/bf02979810
  • Journal Name: Phytoparasitica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.381-392
  • Keywords: Arabidopsis, Peronospora, Race-specific elicitor, Resistance
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

Intercellular washing fluid (IWF) obtained from the susceptible Arabidopsis accession Ws-eds1 inoculated with Peronospora parasitica isolate Emoy-2, contained an elicitor of necrosis with ecotype specificity towards Arabidopsis accessions with particular resistance genes. This elicitor caused necrosis on the highly resistant accessions La-er, Nd-l and partly on Col-5, but not on the susceptible accessions Ws-eds1 and Oy-0. In resistant plants, injection of IWF caused hypersensitive reaction (HR)-like cell collapse which was associated with the accumulation of phenolics and lignin-like material in walls of cells undergoing cell death. The elicitor is sensitive to proteinase K and pronase enzymes, heating and autoclaving but insensitive to periodate oxidation, freezing and thawing, and is not dialyzable. Results suggest that the elicitor is a protein. Fractionation experiments using size-exclusion membranes revealed that elicitor activity has a molecular weight in excess of 100 kDa.