Akademik Veri Yönetim Sistemi
25th ICVF, Amersfoort, Hollanda, 9 - 13 Temmuz 2023, ss.32-33, (Özet Bildiri)
Bluberry mosaic
disease (BMD) was first reported in the
1950s in Michigan but the causal agent was recently
characterized and tentatively named as
blueberry mosaic associated virus (BlMaV) (Thekke-Veetil et al. 2014). The
virus was recently detected in many countries including Turkey (Çağlayan et al.
2015). Although there is not many studies about the natural
transmission ways of BlMaV, Olphidium spp.
stated as potential vector for this virus due to it is the most common vector
for Ophiovirus species. In this study
the ability of Olpidium spp. as a potential vector of BlMaV was evaluated in blueberry fields in Turkey
during spring of 2019-2020. Soil samples were collected from the rhizosphere of
BlMaV infected blueberry plants grown in three different locations in Blacksea
Region of Turkey. These soils from each
location were distributed equally in 12 pots then lettuce, carrot, broccoli and cucumber as trap
plants were planted together with an in vitro
propagated blueberry plants (cv. Bluecrop) in each pot. The experiment was carried out in 3 replications. Eight
weeks after planting, resting spores of Olpidium species were observed in the
roots of 6 lettuce, 1 carrot, 2 broccoli and 6 blueberry plants next to
lettuce. Morphological observations were confirmed by PCR analysis and only Olphidium virulentus was detected and
sequences were deposited in GenBank (MW483635-640; MW48925-28). When all trap plants and adjacent blueberry saplings were tested by using primers specific to a nucleocapsid protein (NP) gene
of BlMaV (Isogai et al. 2016), it was detected only in
lettuce plants among trap plants and in blueberry plants planted next to
lettuce. Since lettuce was found as a good host for both Olpidium and BlMaV,
the experiment was repeated with 6 replications using only lettuce and
blueberry combination and all plants were tested by using different primers
amplifying RNA1, RNA2 and RNA3 of BlMaV.
Thus, 18 blueberry and 13 lettuce isolates for RNA3 (MW422205-234), one blueberry and one lettuce isolate for
RNA2 (MZ513461-462) and one blueberry isolate for RNA1 (OK040161) of BlMaV were
amplified, sequenced and submitted to the GenBank. This study confirmed that O. virulentus plays an efficient role in the transmission of BlMaV
especially if there is good hosts like lettuce for this fungi around blueberry
fields.