Biodiesel production from canola oil using immobilized lipase

YÜCEL Y., Tekeli Y.

Energy Education Science and Technology Part A: Energy Science and Research, cilt.27, sa.2, ss.337-346, 2011 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: Energy Education Science and Technology Part A: Energy Science and Research
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.337-346
  • Anahtar Kelimeler: Biodiesel, Canola oil, Celite 545, Lipase immobilization, Transesterification
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

In the present work, lipase (triacylglycerol hydrolase, EC 3.1.1.3) Novozyme 388 was immobilized onto celite 545 using both adsorption and covalent method. The maximum immobilization yield was obtained as 78.50% when lipase immobilized by covalent method and the highest specific activity was 5.81 U/mg protein for adsorption method. The properties of the support and immobilized derivative were evaluated by fourier transform infrared spectroscopy (FTIR). Immobilized enzyme was used for biodiesel production by transesterification of crude canola oil and methanol. Maximum methyl esters yield was obtained as 98%. Celite 545 activated with polygluturaldehyde proved to be stable after even 10 reuses with each batch of 6 h and lost little activity when was subjected to repeated use. However, the immobilized lipase without polyglutaraldehyde on support material was lost its whole activity after five repeated reuses. © Sila Science.