Production of rhamnolipid (a biosurfactant) using free and immobilized cells of Pseudomonas sp Serbest ve tutuklanmış Pseudomonas sp. hücrelerinden ramnolipid (biyosürfektan) eldesi

Sidal U., YILMAZ E. Ş.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.18, sa.2, ss.285-289, 2012 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 2
  • Basım Tarihi: 2012
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.285-289
  • Anahtar Kelimeler: Biosurfactant, Free cells, Immobilized cells, Pseudomonas sp., Rhamnolipid
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

This study presents a method for the production of rhamnolipid, a biosurfactant, by Pseudomonas sp. Pseudomonas sp. cells that were grown in nutrient agar were inoculated into sterile liquid medium. Following an incubation period of 24 h, 2 ml of cells were inoculated into a different liquid medium and the results were obtained at the end of 26 hours incubation time. In our study, the effects of temperature, pH, and glucose concentration on rhamnolipid production were also investigated. Later, the same procedure was applied to immobilized cells that were kept away from the free microorganisms. The production of rhamnolipid by free cells was found to be much higher than that of immobilized cells. Free cells could be used for rhamnolipid production effectively.