The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese {flnser anser)

Sari E. K., KOZLU T., Kocamis H., Deprem T., Asti R., Nazli M.

Revue de Medecine Veterinaire, vol.160, no.4, pp.176-179, 2009 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 160 Issue: 4
  • Publication Date: 2009
  • Journal Name: Revue de Medecine Veterinaire
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.176-179
  • Keywords: ANAE, Goose, Peripheral blood leukocytes, T lymphocytes
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese, 10 month old, were incubated at various pHs (5.0, 5.4, 5.8, 6.2, 6.4, 6.8 and 7.2) for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity was only recorded in mono-cytes and in some lymphocytes (T cells) according a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes in peripheral blood in goose was 53.6 ± 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.