Kinetic properties and storage stability of catalase immobilized on to florisil

ÖZYILMAZ G., Seyhan Tukel S., ALPTEKİN Ö.

Indian Journal of Biochemistry and Biophysics, cilt.44, sa.1, ss.38-43, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 1
  • Basım Tarihi: 2007
  • Dergi Adı: Indian Journal of Biochemistry and Biophysics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.38-43
  • Anahtar Kelimeler: Catalase, Covalent immobilization, Florisil, Relative humidity, Storage stability
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10°C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g -1 and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 × 105 and 2.0 × 104 μmol H2O2, min-1 mg protein-1, respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4°C and 25°C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.