Akademik Veri Yönetim Sistemi
Uluslararası Katılımlı IX. Bitki Koruma Kongresine, Ankara, Türkiye, 3 - 05 Eylül 2025, ss.69, (Özet Bildiri)
To
date, only Ophiovirus vaccinii
(blueberry mosaic associated virus) (BlMaV) has been detected in Vaccinium spp., whose cultivation and
popularity have been increasing over the years in Türkiye. Within the scope of
this study, samples were collected from new blueberry production areas as well
as Blacksea Region where survey studies have already been conducted, and the
presence of possible new viruses and whole genome analyses of known viruses
were performed using High Throughput Sequencing (HTS) and RT-PCR/Sanger sequencing
technologies. For this purpose, the whole genome sequencing of 10 BlMaV
isolates (5 commercial blueberry, 5 wild blueberry) obtained from different Vaccinium species were performed for the
first time in our country. Thus, an important contribution was supplied to the
world literature by reporting 10 whole genome sequences from our country
regarding BlMaV, which was identified in 2014 and has a limited number of whole
genome data are available in the Gene Bank.
YKD analyses also showed the presence of Nepovirus myrtilli (blueberry leaf mottle virus (BLMoV)), as well
as 3 new blueberry viruses, namely Soymovirus
maculavaccinii (blueberry red ringspot virus (BRRV)), Amalgavirus vaccinii (blueberry latent virus (BlLV)) and blueberry
virüs L (BlVL) which is still unclassified in genus Luteovirus. In addition, the genome sequences of some viruses that
have been detected in different hosts and have not yet been reported in
blueberries, such as Cucumovirus CMV (cucumber mosaic virus (CMV)), Deltapartitivirus
duobetae (beet cryptic virus 2), Duamitovirus crpa1 (Beta vulgaris
mitovirus 1), Potyvirus yituberosi (potato virus Y), grapevine
associated narnavirus-1 (unclassified, Narnavirus) and Botrytis cinerea mitoviruses 1-4 (unclassified, Duamitovirus)
were also detected. When the prevalence
of BlMaV and new blueberry viruses detected in this study were investigated,
58% of the 290 blueberry samples tested were found to be infected by BlVL, 23%
by BlMaV, 10% by BLMoV and 9% by BRRV. This study revealed that the most common
blueberry virus in our country is blueberry virus L, which was detected for the
first time in frame of this study. When
the whole genome sequences of BlMaV isolates from our country were compared
with the reference sequences, it showed 90% nucleotide similarity in the RNA1 and
94% in the RNA2 and RNA3. As a result of the phylogenetic analysis, it was
determined that Turkish BlMaV isolates were in the same group with three
Japanese isolates (LC066299, LC066300 and LC066301), and no significant
recombination signal was found among the Turkish isolates.