Akademik Veri Yönetim Sistemi
Renal Failure, cilt.31, sa.7, ss.567-572, 2009 (SCI-Expanded)
Aim. The objective of the present study was to investigate the effect of low-dose erytropoesis-stimulating agents (ESA) on the development of peritoneal fibrosis in chlorhexidine gluconate-induced peritoneal sclerosing rats and to assess the peritoneal tissue levels of MMP-2 and TIMP-2, which may be regarded as factors in the development of peritoneal fibrosis. Subjects and methods. Twenty-four Wistar albino rats were divided into three groups. The control group received 0.9 saline (3 mld) intraperitoneally, the CH group received 3 ml daily injections of 0.1 chlorhexidine gluconate (CH) intraperitoneally, and the CHESA group received 3 ml daily injections of 0.1 CH intraperitoneally and epoetin beta (3 × 20 IUkgweek) subcutaneously. On the twenth-first day, rats were sacrificed, and parietal peritoneum samples were obtained from the left anterior abdominal wall. Pathological samples were examined using Hematoxyline & Eosin (HE) stains. The thickness, vascular proliferation, and inflammation were determined by light microscopy. MMP-2 and TIMP-2 were studied immunohistochemically by monoclonal antibody staining. Results. Inflammation, vascular proliferation, and fibrotic area percentages were not statistically significant between groups. Histopathologically control, CH, CHESA groups peritoneal thickness were 8.02 ± 2.89, 146.74 ± 26.1, and 48.12 ± 16.8 micrometers, respectively. The decrease in thickness of parietal peritoneum in CHESA group was statistically significant when compared to CH. Immunohistochemically, interferon was shown to decrease MMP-2 expression on parietal peritoneum than group CH, but has no effect on TIMP-2. Discussion. Low-dose ESA histopatologically reduces peritoneal fibrosis induced by chlorhexidine gluconate. However, from dosage and duration points of view, we need extended clinical and experimental studies. © 2009 Informa UK Ltd All rights reserved.