Determination of total phenolic and flavonoid content, mineral composition and free radical scavenging activity of Trigonella plicata Trigonella plicata’nın toplam fenol ve flavonoit miktarı, mineral kompozisyonu ve radikal süpürücü aktivitesi


Uras Güngör Ş. S., Güzel S., İLÇİM A., Kökdil G.

Marmara Pharmaceutical Journal, vol.20, no.3, pp.341-345, 2016 (Scopus, TRDizin)

  • Publication Type: Article / Article
  • Volume: 20 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.12991/mpj.20162084302
  • Journal Name: Marmara Pharmaceutical Journal
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.341-345
  • Keywords: Mineral composition, Radical scavenging activity, T. plicata, Toplam phenol and flavonoid
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

The aim of the present study was to determine total phenolic and flavonoid content and free radical scavenging activity and mineral composition of Trigonella plicata Boiss. & Bal. from Turkey. Total phenol, flavonoid content and free radical scavenging activity of the aqueous methanolic extract obtained from seeds was measured spectrophotometrically by Folin-Ciocalteu, the AlCl3 and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The phenolic content was 64.11±0.36 mg gallic acid equivalent (GAE)/g dry weight while the flavonoid content was 51.02±0.056 mg rutin equivalent (RE)/g dry weight. In free radical scavenging assay, inhibition % of the extract (1g/L)was found to be 50.1±0.039. The mineral composition of the seeds were determined by ICP-MS and potassium was main mineral in the seeds. The other main elements, in descending order by quantity, were Ca, P, Mg and Na.