Protective effect of Nigella sativa in an animal model of colon anastomosis with ischemia/ reperfusion injury
International Surgery, vol.103, no.3-4, pp.139-148, 2019 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 103 Issue: 3-4
- Publication Date: 2019
- Doi Number: 10.9738/intsurg-d-15-00301.1
- Journal Name: International Surgery
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.139-148
- Keywords: Anastomosis, Anastomotic leaks, Ischemia/reperfusion injury, Nigella sativa
- Hatay Mustafa Kemal University Affiliated: Yes
Abstract
Objective: Anastomotic leaks are one of the chief complications after gastrointestinal surgery. The aim of this study was to evaluate whether Nigella sativa administration protects against ischemia/reperfusion injury on healing of colonic anastomosis in rats. Method: Thirty male Wistar albino rats, weighing between 200 and 240 g, were used in the study. They were randomly divided into three groups (n ¼ 10 for each group): Anastomosis (group 1), anastomosis and ischemia/reperfusion injury (group 2), and treatment group of anastomosis, ischemia/reperfusion injury, and Nigella sativa (group 3). After 7 days, serum, plasma, and colonic tissue were obtained and then all rats were sacrificed. Tissue and serum level of total oxidant status, total antioxidant status, total thiol levels, hydroxyproline, interleukin-6, and TNF-alpha were determined and specimens were histopathologically evaluated. Results: In the Nigella sativa treated rats, serum hydroxyproline levels were significantly higher, while tissue levels were significantly lower than those seen in group 1 and group 2 (P ¼ 0.007, P ¼ 0.01, respectively). In the Nigella sativa group, the serum levels of TNF-a were significantly lower than those seen in group 1 and 2 (P ¼ 0.001). Also, in group 3, the tissue IL-6 level was significantly higher than that seen in group 1 and group 2 (P ¼ 0.009). The histopathologic analysis showed less edema and inflammatory cell infiltration in the Nigella sativa treated group, as well as a statistically significant difference according to the Chiu classification (P, 0.05). Conclusion: The results of this study indicate that Nigella sativa has a protective and therapeutic effect against ischemia/reperfusion injury on the healing of colonic anastomosis in rats.