The effect of dexpanthenol on experimentally induced ovarian ischaemia/reperfusion injury: a biochemical and histopathological evaluation

SOYLU KARAPINAR O., PINAR N., ÖZCAN O., Atik Doğan E., Bayraktar S., Şahin H., ...More

Archives of Gynecology and Obstetrics, vol.295, no.3, pp.777-784, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 295 Issue: 3
  • Publication Date: 2017
  • Doi Number: 10.1007/s00404-017-4287-y
  • Journal Name: Archives of Gynecology and Obstetrics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.777-784
  • Keywords: Dexpanthenol, Ischaemia/reperfusion, Ovarian torsion, Oxidative stress
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

Objective: The aim of this study was to evaluate the effect of two different doses of dexpanthenol (Dxp) onexperimentally induced ovarian ischaemia/reperfusion (I/R) injury ina rat model. Study design: Forty female rats were randomly divided into fivegroups: Group 1: sham operation; Group 2: 3-h ischaemia; Groups 3: 3-h ischaemia, 3-h reperfusion (I/R); Group 4: I/R + 300 mg/kg Dxp intraperitoneally (i.p) Group 5: I /R + 500 mg/kg Dxpi.p. Total anti-oxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), tissue malondialdehyde (MDA) and activities of glutathione peroxidase and catalase were calculated. Ovarian tissue damage was assessed using a histopathological scoring system. Results: The TOS and OSI values were significantly lower in Group 5, as compared to Groups 2 and 3 (p < 0.05). The MDA levels in Group 1 and Group 5 were significantly lower than those in Group 3 (p < 0.05). CAT and GSH-Px activity was higher in Group 5 than in Group 2 and Group 3 (p = 0.00). Tissue damage scores were elevated in all the groups compared with sham group, but the treatment with the different doses of Dxp before reperfusion ameliorated the tissue damage scores. Conclusion: The results showed that Dxp reduced ovarian I/R injury.