Latin American Applied Research, cilt.53, sa.2, ss.119-123, 2023 (SCI-Expanded)
The current work was conducted in or-der to explore the influence of ultrasound times and amplitudes on the characterization (droplet size, tur-bidity, and emulsion stability) and lipid oxidation of whey protein nanoemulsions (WPCON). Ultrasound (US) application was employed using a VC-750 ultra-sonic power equipment with the frequency of 20 kHz at various times (10, 20, and 30 minutes at 50% am-plitude) and amplitudes (60%, 80%, and 100% for 5 min). The outcomes exhibited that the US process have a significant impact on both functional proper-ties and lipid oxidation (p<0.05). The smallest droplet size was obtained for the samples exposed to 30 min US at 100% amplitude (608.3 nm). WPCON samples treated at 100% amplitude showed smaller droplet sizes compared to the other samples at 60% and 80% amplitudes. While the droplet size of WPCON sam-ples treated with 10 min showed biggest droplet size (895.2 nm), samples treated with 30 min showed the smallest droplet size (795.1 nm) in terms of ultra-sound times. There is a positive relationship between the WPCON of droplet size and turbidity. All US-treated WPCON samples exhibited less turbidity and smaller droplet size where the control WPCON sam-ples demonstrated most turbid structure with the big-gest droplet sizes. In addition, the best emulsifying properties (EAI: 255.08 m2 /g and ESI: 43 min) was obtained for the samples exposed to 30 min US at 100% amplitude (US310). The same sample showed the least lipid oxidation during 7 days of storage