Methods for lipase immobilization and their use for biodiesel production from vegetable oil

YÜCEL Y., Demir C., Dizge N., Keskinler B.

Energy Sources, Part A: Recovery, Utilization and Environmental Effects, cilt.36, sa.11, ss.1203-1211, 2014 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 11
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/15567036.2010.545805
  • Dergi Adı: Energy Sources, Part A: Recovery, Utilization and Environmental Effects
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1203-1211
  • Anahtar Kelimeler: alcoholysis, biodiesel, lipase immobilization, Lipozyme TL-100L, Novozyme 388, transesterification
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

In the present work, two different lipases (triacylglycerol hydrolase, EC 3.1.1.3), Lipozyme TL-100L and Novozyme 388, were immobilized onto three different low-cost supports using both adsorption and covalent method: celite 545, silica gel, and styrene-divinylbenzene copolymer. The maximum immobilization yield was obtained as 79.0% for Lipozyme TL-100L and the highest specific activity was 6.5 U/mg protein for Novozym 388. The properties of the support and immobilized derivatives were characterized by Fourier transform infrared spectroscopy. Maximum methyl esters yield was obtained as 98.3%. The lipases, which are immobilized by covalently, proved to be stable after even 10 repeated reuses. Copyright © 2014 Taylor & Francis.