Investigation of antioxidant effect of silibinin molecule on U-2 OS cells induced by hydrogen peroxide


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GÜNDÜZ K., ECEVİT H., Urhan Küçük M., DIRICAN E.

Annals of Medical Research, cilt.29, sa.5, ss.502-508, 2022 (Hakemli Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.5455/annalsmedres.2021.09.558
  • Dergi Adı: Annals of Medical Research
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.502-508
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Aim: Silibinin is one of the active constituent of silymarin, an extract of the Silybum marianum L. seeds. There are previous studies that have reported the antioxidant, antiinflammatory, anti-apoptotic and anti-carcinogenic effects of silibinin. However, the antioxidant effect of silibinin on bone cancer have been not evaluated before. In our study, we aimed to evaluate the effect of silibinin on U-2 OS cell proliferation and oxidative stress parameters catalase enzyme activity and MDA level. Materials and Methods: U-2 OS cells were exposed to H2O2 (0-800 µM H2O2, for 24 and 48 hours) to form an oxidative damaged cell model. Moreover, the cells were exposed to silibinin (0-100 µM) for 24 hours. Malondialdehyde (MDA) level and antioxidant enzyme catalase (CAT) activity were determined by spectrophotometric analysis. Results: CAT activity and MDA level were observed to be higher in both H2O2 exposure groups compared to control group (p<0.05). Moreover, CAT activity and MDA level were observed to be lower in 500 µM H2O2+silibinin groups in comparation with 500 µM H2O2 group (p<0.05). Similarly, MDA level was determined to be lower in 650 µM H2O2+10 µM silibinin group in comparation to 650 µM H2O2 group (p<0.05). Conclusion: In conclusion, it was observed that silibinin molecule has anti-proliferative effect and decreases catalase enzyme activity and MDA level in a concentration dependent manner.