Akademik Veri Yönetim Sistemi
Veterinary Medicine and Science, cilt.12, sa.1, 2026 (SCI-Expanded, Scopus)
Antimicrobial resistance in various infectious agents represents a public health threat. Therefore, the use of antimicrobials reveals new resistance mechanisms, and these resistance mechanisms are spreading globally. In this study, the aim was to isolate Escherichia coli from samples by conventional culture method and to identify the E. coli isolates obtained by PCR analysis. As a result of the culture method, 120 of 300 samples (40%) were isolated as suspected E. coli. However, the detection of the trpA gene only in 90 (75%) of the isolates allowed for the confirmation that it was, in fact, E. coli. According to PCR results, a total of 10 (11%) isolates were identified as E. coli subgroups. Eight (80%) of these isolates were found to be E. coli O157:H7 positive, while two (20%) were found to be E. coli O157 positive. In addition, five (50%) isolates were found to be H7 positive. Antimicrobial effect (Enzyme-Linked Immuno Sorbent Assay) was determined using the ELISA method. The results show that the CuONPs at 0.085 µg/mL had antibacterial properties against all strains. Antibiotic susceptibility was tested using the disc diffusion method. Nine (10%), 6 (6%), 28 (31%), 24 (26%), 5 (5%), 8 (8.8%), 4 (4%) and 6 (6%) tetracyclines, erythromycin, gentamicin, azithromycin, imipenem, ampicillin and nalidixic acid were found to be resistant, respectively. According to the results of the analysis, 1 and mcr 5 gene levels were found in 10 (11%) and 2 (2.2%) isolates, respectively. Also, in the isolates, E. coli O157:H7 had mcr 1 and mcr 5 genes in levels 3 (37.5) and 2 (25%). Nonetheless, 2 (100%) E. coli O157 isolates carried the mcr 1 gene, while 4 (80%) H7 isolates carried the mcr 1 gene. In this study, virulence genes were detected in all E. coli O157:H7 isolates. According to Congo red agar, two E. coli isolates were found to have strong biofilm formation ability, while three E. coli isolates were found to have moderate biofilm formation ability. The results of this study, due to the antimicrobial resistance, virulence genes and biofilm formation abilities of the strains, emphasize that these strains pose a significant risk to public health because they are associated with foodborne diseases and that this situation poses a danger of spreading the resistance gene in the environment.