Akademik Veri Yönetim Sistemi
Journal of Basic Microbiology, cilt.51, sa.2, ss.136-146, 2011 (SCI-Expanded)
A novel moderately thermophilic, Gram-positive staining, rod-shaped, spore-forming, motile, facultative anaerobic, and α-glucosidase producing strain A343T, was isolated from a high temperature well-pipeline sediment sample in Salavatli province of Aydin, Turkey. Growth was observed at 37-69 oC (optimum 60 °C), at pH 5.5-9.5 (optimum 8.0-9.0) and at salinities from 0 to 4.5% (w/v) (optimum 2%). Strain A343T was able to grow on a wide range of carbon sources. Gelatin and starch utilization, amylase, catalase and oxidase activities, reduction of nitrate to nitrite were all positive. The G+C content of the genomic DNA was 45.1 mol%. The major menaquinone was MK-7. The dominant cellular fatty acids were: iso-C15:0, C16:0, and iso-C17:0. The phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain A343T belonged to the genus Anoxybacillus. The 16S rRNA gene sequence similarity between strain A343T and the type strains of recognized Anoxybacillus species was ranged from 95.8 to 99.4%. DNA-DNA hybridization revealed low homology with its closest relative Anoxybacillus kamchatkensis (49.7%). In addition to the total cell protein profiles, the Rep-PCR and the intergenic 16S-23S rRNA gene fingerprinting profiles differentiated strain A343T from all of the reference Anoxybacillus species used. Based upon phenotypic, phylogenetic and chemotaxonomic evidence, strain A343T was assigned to a new species within the genus Anoxybacillus, A. salavatliensis sp. nov. (The type strain A343T= DSM 22626T = NCIMB 14579T). The 16S rRNA gene nucleotide sequence of strain A343T is available in the GenBank database under the accession number - EU326496. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.