Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design

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Yağız E., ÖZYILMAZ G., ÖZYILMAZ A. T.

Natural and Engineering Sciences, cilt.7, sa.1, ss.22-33, 2022 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.28978/nesciences.1098655
  • Dergi Adı: Natural and Engineering Sciences
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.22-33
  • Anahtar Kelimeler: biosensor, Carbon paste electrode, Glucose Oxidase, polyaniline, Response Surface Methodology
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). For this, State Ease Design Expert 8.0.7.1. (Serial Number: 0021-6578) software software was used applying Optimal Design. By using data obtained, Design Expert software suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test.