Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm
Israeli Journal of Aquaculture - Bamidgeh, cilt.63, sa.1, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 63 Sayı: 1
- Basım Tarihi: 2011
- Doi Numarası: 10.46989/001c.20596
- Dergi Adı: Israeli Journal of Aquaculture - Bamidgeh
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Anahtar Kelimeler: cryopreservation, Ctenopharyngodon idella, extender, fertilization, glycerol, grass carp, semen
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
This research investigated the effect of adding glycerol to different ionic extenders on motility and fertilizing ability of frozen-thawed sperm of grass carp (Ctenopharyngodon idella) under hatchery conditions. Semen was collected by abdominal stripping from adult males and diluted to a ratio of 1:3 in Kurokura I or Kurokura II extender containing 10%, 15%, or 20% glycerol. Diluted semen was packaged in 0.5-ml straws and left to equilibrate for 30 min at 4°C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196°C). Straws were thawed in a water bath at 30°C for 10 s to determine the post-thaw motility and movement duration. The spermatozoa:egg ratio in fertilization experiments was 1 x 105:1. The highest mean fertilization rate (95.63±0.52; p>0.05) was obtained with semen frozen in Kurokura I extender containing 10% glycerol. Results indicate that grass carp sperm can be successfully cryopreserved with ionic extenders containing glycerol at 10-20%.