Akademik Veri Yönetim Sistemi
Cahiers de Biologie Marine, cilt.62, sa.4, ss.321-330, 2021 (SCI-Expanded)
The epidermal mucus of two ray species Dasyatis pastinaca and Raja miraletus was analyzed to identify biochemical compounds and explore their antimicrobial activities in order to point out their potential for human health-related applications. Epidermal mucus samples were extracted as acidic, crude and aqueous. Gas chromatography and gas chromatography-mass spectrometry (GC/MS) analyses were performed to determine the biochemical compounds of the mucus. The antibacterial and antifungal activities of the extracts obtained from mucus were analysed using the spectrophotometric broth microdilution method against bacterial (Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis and Enterococcus faecalis) and fungal (Candida albicans and Candida parapsilosis) strains. Gas chromatography and gas chromatography-mass spectrometry (GC/MS) analyses revealed the existence of several hydrocarbon-derived compounds and polycyclic aromatic hydrocarbons. In terms of antimicrobial activity, the highest detected MIC (minimum inhibitory concentration) value was 9.78 µL for the acidic extract of R. miraletus epidermal mucus (RmAC) against E. faecalis, while the lowest MIC value was 80.88 µL for the acidic extract of R. miraletus epidermal mucus (RmAC) against E. coli. The extracts of epidermal mucus from D. pastinaca and R. miraletus showed antimicrobial activities against all the used pathogens with varying degrees of efficiency and thus may be evaluated as a potential source of marine-derived antimicrobial components for biomedical purposes.