Akademik Veri Yönetim Sistemi
APPLIED FRUIT SCIENCE, cilt.66, sa.6, ss.2347-2353, 2024 (SCI-Expanded)
The grape erineum mite, Colomerus vitis, can cause extensive damage to grape bunches, and can lead to leaves lacking normal development and yield being reduced. The mite is also a vector of grapevine Pinot gris virus (GPGV), which is an emerging virus reported in many countries in Europe and also in T & uuml;rkiye. In this study, C. vitis individuals collected from buds of GPGV-infected vines were characterized through morphological and molecular techniques and their role in GPGV transmission was investigated. Morphological identification studies revealed that the eriophyid mites collected from GPGV-infected grapevines were C. vitis. PCR analysis using mitochondrial cytochrome oxidase subunit I (COI) gene-specific primers confirmed the morphological observations and a 650-bp fragment was successfully amplified. Five samples were selected and sequenced. All Turkish C. vitis isolates were grouped together and their intraspecies diversity was found to be lower than other world isolates. The presence of GPGV in C. vitis collected from GPGV-infected grapevines was detected by RT-PCR analysis, showing an infection rate of 1.38% (6 individuals/434 tested mites). Experimental transmission trials of GPGV to healthy grapevines using C. vitis collected from GPGV-infected grapevines were performed. Although no symptoms were observed in eriophyid inoculated healthy vines during 1-year observations, RT-PCR analyses showed that two out of ten inoculated vines were infected by GPGV. This is the first report on the molecular identification of C. vitis using the COI gene and showing the potential role of the pathogen in GPGV transmission in Turkiye.