Background: Screening aimed at hepatitis B reactivation risk is recommended in patients using immunosuppressive drugs. The aim of this study was to investigate the results of hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis A virus (HAV) retrospectively in patients referred to the infectious diseases clinic before immunosuppressive treatment. Materials and Methods: In our study, 148 patients who were referred to our infectious disease clinic before immunosuppressive therapeutic usage between January 2018-December 2018. Patients files and hospital automation sysytem were analyzed for Hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (AntiHBs), hepatitis B core protein antibody (Anti -HBc IgG), anti-hepatitis C virus antibody (Anti-HCV) and antihepatitis A virus antibody (anti -HAV) retrospectively. Results: HbsAg positivity rate was 4.1%, Anti-HBs positivity rate was 31.8%, Isolated Anti-HBc IgG positivity rate was 1.4% and Anti-HBs was not detected in two patients. Anti-HCV positivity was detected as 1.4%. Anti-HAV was not examined in 79.7% of the patients, 12.2% was determined to have positive Anti-HAV and 8.1% negative Anti-HAV. Conclusions: Since some screenings are incomplete despite screening performed for the patients, we recommend that physicians who follow patients receiving immunosuppressive therapy should be trained and raise awareness of HBV infection.