Effects of Melatonin and Coenzyme Q<sub>10</sub> Added to Extenders on Spermatological Properties in Cryopreservation of Epididymal Rat Sperm
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.76, sa.3, ss.9785-9796, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 76 Sayı: 3
- Basım Tarihi: 2025
- Dergi Adı: JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
- Sayfa Sayıları: ss.9785-9796
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
With the use of advanced genome editing technology, the production of genetically modified mutant rats worldwide, and the successful freezing and storage of their gametes, have become important in biotechnology. In the present study, the effects of different doses of melatonin (0.5mM, 1mM, 2mM) and coenzyme Q(10 )(25 mu M, 50 mu M, 75 mu M) added to the spermatozoa freezing medium containing 8% lactose monohydrate, 23% egg yolk (EY), and 10% trisaminomethane on the freezability of rat spermatozoa were investigated. After freezing and thawing, motility, dead spermatozoa rate, plasma membrane integrity, abnormal acrosome rate, motility longevity, apoptotic and necrotic cell rates were evaluated. Compared to the control group (12.90 +/- 1.34%), the highest motility value was found in the 1mM Melatonin group (24.58 +/- 1.68%) and 25 mu M Coenzyme Q(10) group (24.23 +/- 1.95%) (p<0.01). The lowest dead spermatozoa rate was determined in the 1mM Melatonin group (63.08 +/- 4.89%) compared to the control group (75.47 +/- 2.27%) (p<0.01). Plasma membrane integrity rates were higher in the 1mM Melatonin (35.50 +/- 1.82%) and 25 mu M Coenzyme Q(10) (31.31 +/- 1.93%) groups compared to the control group (18.21 +/- 1.36%) (p<0.01). The best motility longevity was observed in the 1mM melatonin and 25