Determination of ethyl glucuronide in fingernails by LC/MS-MS

Keten A., Zeren C., ARSLAN M. T., DAĞLIOĞLU N., Karanfil R., Şen B. B. Ş.

Romanian Journal of Legal Medicine, vol.21, no.1, pp.67-72, 2013 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.4323/rjlm.2013.67
  • Journal Name: Romanian Journal of Legal Medicine
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.67-72
  • Keywords: Alcohol intake, Ethyl glucuronide, Fingernails, LC/MS/MS
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

Introduction: Ethyl glucuronide (EtG) is a biomarker used for detection of alcohol consumption. Fingernail is a keratinous tissue capable of accumulating a number of biomarkers and metabolites of toxic agents. Therefore, nails are used especially for determination toxic exposure in forensic analyses. In this study, we aimed to demonstrate EtG in fingernail tissue and show its correlation with alcohol intake behavior. Materials and Methods: Michigan Alcohol Screening Test (MAST) was performed to the individuals participated in the study. Sixteen fingernails were obtained from each individual. Afterwards, EtG analysis was carried out in fingernail tissue using LC/MS/MS. Results: Michigan Alcohol Screening Test revealed that 4 participants showed non-hazardous drinking behavior and 8 cases were alcohol abusers, while 4 cases verbally stated they were abstainers. On the analyses, EtG was found between EtG level of