Dietary high calories from sunflower oil, sucrose and fructose sources alters lipogenic genes expression levels in liver and skeletal muscle in rats


ÖZKAN H., Yakan A.

Annals of Hepatology, vol.18, no.5, pp.715-724, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 5
  • Publication Date: 2019
  • Doi Number: 10.1016/j.aohep.2019.03.013
  • Journal Name: Annals of Hepatology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.715-724
  • Keywords: Fatty liver, Fructose, Gene expression, Lipogenesis, Metabolism of skeletal muscle
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

Introduction and Objectives: The objectives of this study were to investigate the underlying mechanism of PPARα, LXRα, ChREBP, and SREBP-1c at the level of gene and protein expression with high-energy diets in liver and skeletal muscle. Materials and methods: Metabolic changes with consumption of high fat (Hfat), high sucrose (Hsuc) and high fructose (Hfru) diets were assessed. Levels of mRNA and protein of PPARα, LXRα, ChREBP, and SREBP-1c were investigated. Body weight changes, histological structure of liver and plasma levels of some parameters were also examined. Results: In Hfru group, body weights were higher than other groups (P < 0.05). In liver, LXRα levels of Hsuc and Hfru groups were upregulated as 1.87 ± 0.30 (P < 0.05) and 2.01 ± 0.29 (P < 0.01). SREBP-1c levels were upregulated as 4.52 ± 1.25 (P < 0.05); 4.05 ± 1.11 (P < 0.05) and 3.85 ± 1.04 (P < 0.05) in Hfat, Hsuc, and Hfru groups, respectively. In skeletal muscle, LXRα and SREBP-1c were upregulated as 1.77 ± 0.30 (P < 0.05) and 2.71 ± 0.56 (P < 0.05), in the Hfru group. Protein levels of ChREBP (33.92 ± 8.84 ng/mg protein (P < 0.05)) and SREBP-1c (135.16 ± 15.57 ng/mg protein (P < 0.001)) in liver were higher in Hfru group. In skeletal muscle, LXRα, ChREBP and SREBP-1c in Hfru group were 6.67 ± 0.60, 7.11 ± 1.29 and 43.17 ± 6.37 ng/mg, respectively (P < 0.05; P < 0.01; P < 0.05). The rats in Hfru group had the most damaged livers. Conclusion: Besides liver, fructose consumption significantly effects skeletal muscle and leads to weight gain, triggers lipogenesis and metabolic disorders.