Research Information System
Kafkas Universitesi Veteriner Fakultesi Dergisi, vol.23, no.1, pp.63-67, 2017 (SCI-Expanded)
In this study, it was aimed to determine the prevalence of extended spectrum β-lactamase (ESBL) and/or AmpC type β-lactamase (AmpC) producing Escherichia coli from cattle in Hatay. For this purpose, 312 rectal swabs samples were collected from apparently healthy cattle. ESBL production was phenotypically investigated by disc combination method and double disc synergism test and β-lactamase genes (blaCTX-M, blaCMY-2, blaSHV, blaOXA, and blaTEM) and plasmid mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS and aac(6’)-Ib) were screened by polymerase chain reaction (PCR) and subsequent sequence analysis. Antimicrobial susceptibility of the isolates were determined using disc diffusion method and their phylogenetic groups were also searched by PCR. Twenty six (8.3%) isolates were found to be ESBL producer by phenotypic tests. The following ESBL/AmpC genes were detected: blaCTX-M-15 (n= 12), blaCTX-M-1 (n=11), blaCTX-M-3 (n=2), and blaCMY-2 (n=1). PMQR genes were detected in 11 (42.3%) ESBL producing E. coli isolates and these isolates were only positive for aac(6’)-Ib-cr and qnrS1 genes. Twenty two (84.6%) of the isolates exhibited multidrug resistance (MDR) phenotype. ESBL/AmpC producing E. coli isolates were observed to be belonged to B1 (50%), A (34.6%) and D (15.4%) phylogroups. This study was the first to describe the presence of CTX-M-15, CTX-M-3, CTX-M-1 and CMY-2 producing E. coli in cattle in Turkey and the co-existence of aac(6’)-Ib-cr and qnrS1 genes in some isolates.