Akademik Veri Yönetim Sistemi
Acta Horticulturae, cilt.657, ss.127-132, 2004 (Scopus)
Field inspection and sample collection were carried out in mother plant blocks which are also varietal collection belonging to the Ministry of Agriculture and the Universities in Eastern Mediterranean Region of Turkey during early spring and in autumn in 2002. Samples (shoots and leaves) were collected from symptomatic plants in the orchards. 48 samples (3 almond, 9 apricot, 3 nectarine, 10 peach, 11 plum, 12 sweet cherry) having different symptoms were collected and tested for the presence of Apple chlorotic leaf spot trichovirus (ACLSV), Apple mosaic ilarvirus (ApMV), Cherry leaf roll nepovirus (CLRV), Prune dwarf ilarvirus (PDV), Prunus necrotic ringspot ilarvirus (PNRSV) and Plum pox potyvirus (PPV) by using DAS-ELISA. According to serological assays, the most common virus was PDV. It was followed by PNRSV and ACLSV. The reverse transcription-polymerase chain reaction (RT-PCR) assays were performed for investigation of PDV, PNRSV and PPV in the samples. The following viruses were identified by ELISA and RT-PCR assays: ACLSV (1 sample), CLRV (1 sample), PDV (5 samples), PNRSV (2 samples). Mix infections of ACLSV+PDV (1 sample) and PDV+PNRSV (2 samples) were also found. No PPV-infected samples were detected from the samples collected from mother plant blocks in Eastern Mediterranean Region of Turkey.