Akademik Veri Yönetim Sistemi
Uluslararası Katılımlı IX. Bitki Koruma Kongresine, Ankara, Türkiye, 3 - 05 Eylül 2025, ss.68, (Özet Bildiri)
It is known that more than twenty viruses identified in daffodils cause significant yield and quality losses. Although there are many studies on viruses in daffodils in the world, studies on this subject in our country are very limited. This study aimed to determine the prevalence and molecular characteristics of potyviruses infecting daffodils cultivated in the Eastern Mediterranean Region of Türkiye. In this study, 224 symptomatic and asymptomatic daffodil samples collected from commercial gardens and natural plantations in daffodil growing areas in the Eastern Mediterranean Region were tested against potyviruses by DAS-ELISA and RT-PCR analyses. When the collected samples were tested by DAS-ELISA method, 80 samples were found positive for potyviruses, while 84 samples were found positive in RT-PCR analyses using universal primers for Potyvirus genus. In BLAST analyses performed on 10 samples that were found positive with NIb primers and sent for sequence analysis, narcissus yellow stripe virus (NYSV) (Potyvirus narcissus) was detected in 6 samples and narcissus late season yellows virus (NLSYV) (Potyvirus narcissuslineae) was detected in 4 samples. When species-specific primers were used for NYSV and narcissus degeneration virus (NDV) (Potyvirus narcissusdegeneris), 76 and 18 samples among tested 224 samples were found infected by these viruses, respectively. When 224 tested samples were evaluated for mixed infections, 14 samples were detected to be infected with NYSV-NDV, 4 samples with NYSV-NLSYV and 1 sample with NYSV-NDV-NLSYV. When 84 samples found positive with NIb primers were tested with species-specific primers for narcissus latent virus (NLV) (Macluravirus narcissi) and narcissus mosaic virus (NMV) (Potexvirus narcissi), these viruses were not detected in any sample.In phylogenetic analyses comparing DNA sequences of the NIb gene region of Potyvirus isolates detected in our daffodil samples with isolates from around the world, some of the Antakya and Tarsus isolates were in the same main branch with global NYSV isolates, while some of the Antakya isolates were in the same main branch with NLSYV isolates from different countries, supporting the successful use of the NIb gene region in the distinction of Potyvirus species. When our NYSV isolates phylogenetically compared with global isolates, 3 phylogenetic groups were identified and it was determined that our all isolates were in the first group. They showed a high similarity with isolates from Japan and China. In the phylogenetic analyses of NDV isolates from our country, they were in the first group in the tree divided into three main phylogenetic groups, showing a very high similarity (99.5%) among themselves.With this study, the detection and characterization studies of narcissus viruses were first time carried out using serological and molecular methods in our country. It has been shown that NYSV, NDV and NLSYV were quite common in commercially cultivated narcissus, but no virus was detected in wild narcissus.