Subklinik inek mastitis'lerinden izole edilen staphylococcus aureus suşlarinin koagulaz gen polimorfizmi


ASLANTAŞ Ö., DEMİR C., TÜRÜTOĞLU H., Cantekin Z., EGÜN Y., DOĞRUER G.

Turkish Journal of Veterinary and Animal Sciences, vol.31, no.4, pp.253-257, 2007 (SCI-Expanded, Scopus, TRDizin) identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 4
  • Publication Date: 2007
  • Journal Name: Turkish Journal of Veterinary and Animal Sciences
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.253-257
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.
Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction (PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar, çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir