Role of epigallocatechin gallate on in vitro model of methylglyoxal-induced amyloidogenesis


KÜÇÜKGÜL A., İŞGÖR M. M., CELLAT M., Ata H.

Asian Journal of Pharmaceutics, cilt.10, sa.3, 2016 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 3
  • Basım Tarihi: 2016
  • Dergi Adı: Asian Journal of Pharmaceutics
  • Derginin Tarandığı İndeksler: Scopus
  • Anahtar Kelimeler: Amyloidogenesis, Epigallocatechin gallate, In vitro, Methylglyoxal
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Introduction: The study aimed to investigate the potential effects of epigallocatechin gallate (EG) on the reflux of methylglyoxal (MG)-induced amyloidogenesis in human glioblastoma (U87) cells. Materials and Methods: The effective concentrations of MG and EG were investigated via Trypan blue test. Glyoxalase-1 (GLO-1), β-amyloid precursor protein (βAPP), and Caspase 3 (Cas 3) expression levels were determined by quantitative real-time polymerase chain reaction and intracellular glutathione (GSH) contents of cells were measured. Results: MG at 250 μM reduced viable cells by 33.4% as compared to control group. However, 5 μM EG pre-treatment before MG prevented 22.4% of the cell loss caused by MG (P ≤ 0.05). MG stimulated βAPP and Caspase 3 levels by 4.13- and 3.46-fold; however, EG pre-treatment inhibited these increases by 1.76 and 3.09, respectively. In addition, EG pre-treatment increased GLO-1 levels by 3.71-fold and GSH levels by 2.30-fold according to MG group. Conclusion: EG demonstrated protective effect against cell death on U87 cells by suppressing amyloidogenic factors and apoptotic stimuli induced by MG.