Research Information System
Turkish Journal of Agriculture and Forestry, vol.26, no.6, pp.343-348, 2002 (Scopus)
Broad bean (Vicia faba) cotyledons that were inoculated artificially with fungal pathogens or exposed to various abiotic agents were analysed for phytoatexin production. Biotic elicitors, such as Botrytis cinerea and B. allii, and abiotic elicitors, such as ultraviolet (UV) radiation (254 nm) and freezing-thawing, were used to induce phytoalexin accumulation. Wyerone and other wyerone derivatives were the major phytoalexins responding in broad bean cotyledons. The quantities of wyerone within elicitortreated tissues were examined by thin layer chromatography. The highest amount of wyerone was induced by B. cinerea (943 μg/g fresh weight). Treatment of cotyledons with UV radiation (452 μg/g f.wt), B. alli (325 μg/g f.wt) and freezing-thawing (288 μg/g f.wt) also caused considerable activation of the phytoalexin synthesis. Cell necrosis and wyerone accumulation were closely associated, and the highest concentration of wyerone was in tissue bearing brown lesions. Only very low concentrations of wyerone accumulated at sites of mechanical damage. The results indicate that the presence of both damaged and healthy tissues is necessary for phytoalexin production.