Comparison of renal lesions in cats and dogs using pathomorphological and ımmunohistochemical methods
Biotechnic and Histochemistry, cilt.94, sa.2, ss.126-133, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 94 Sayı: 2
- Basım Tarihi: 2019
- Doi Numarası: 10.1080/10520295.2018.1522670
- Dergi Adı: Biotechnic and Histochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.126-133
- Anahtar Kelimeler: Chronic disease, epithelial-mesenchymal transformation, fibrosis, kidney, nephritis, nephrosis
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
We investigated the pathogenesis of chronic renal fibrosis in cats and dogs using immunohistochemistry. We used the avidin-biotin complex peroxidase (ABC-P) method with antibodies against transforming growth factor-β1, cytokeratin, E-cadherin, S100A4, alpha-smooth muscle actin, vimentin and nestin to determine whether tubule epithelial cells had undergone epithelial-mesenchymal transformation (EMT) that resulted in loss of epithelial cells and an increased number of mesenchymal cells. Although nephrosis was more common in dogs, nephritis was more common in cats; these pathologies developed in both kidneys. We found that EMT participated in the pathogenesis of renal fibrosis in both dogs and cats.