Comparison of renal lesions in cats and dogs using pathomorphological and ımmunohistochemical methods

KUTLU T., Alcigir G.

Biotechnic and Histochemistry, cilt.94, sa.2, ss.126-133, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 94 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/10520295.2018.1522670
  • Dergi Adı: Biotechnic and Histochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.126-133
  • Anahtar Kelimeler: Chronic disease, epithelial-mesenchymal transformation, fibrosis, kidney, nephritis, nephrosis
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

We investigated the pathogenesis of chronic renal fibrosis in cats and dogs using immunohistochemistry. We used the avidin-biotin complex peroxidase (ABC-P) method with antibodies against transforming growth factor-β1, cytokeratin, E-cadherin, S100A4, alpha-smooth muscle actin, vimentin and nestin to determine whether tubule epithelial cells had undergone epithelial-mesenchymal transformation (EMT) that resulted in loss of epithelial cells and an increased number of mesenchymal cells. Although nephrosis was more common in dogs, nephritis was more common in cats; these pathologies developed in both kidneys. We found that EMT participated in the pathogenesis of renal fibrosis in both dogs and cats.