Akademik Veri Yönetim Sistemi
Turkiye Klinikleri Journal of Medical Sciences, cilt.41, sa.4, ss.470-477, 2021 (Scopus)
Objective: Inflammation is the primary biological response of immune system against infection, injury, and irritation, but when it is not curbed, it can lead to autoimmune and neurodegenerative diseases or cancer. Myrtus communis L. (MC) is an aromatic plant raised in the re-gion of Mediterranean Sea and bearing several pharmacological activities. We aimed in this study to investigate anti-inflammatory effects of MC essential oil that is often utilized by the local population. Material and Methods: Cell culture experiments were conducted with healthy bronchial epithelial lung cell line (BEAS-2B). The cells were grouped in three as follows; non-treated control, interleukin-1 beta (IL-1β) induced inflammatory cells, and MC-treated inflammatory cells. Non-cytotoxic concentrations of MC were determined with MTT assay. The gene expression levels of IL-6, IL-10, inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), and nuclear factor kappa b (NFκB) were analyzed with reverse transcriptase-quantitative polymerase chain reaction. The protein levels of IL-6, IL-10, iNOS, and TNF-α were detected with ELISA. Results: The elevated mRNA levels of IL-6, IL-10, and NFκB in IL-1β-induced inflammatory cells significantly reduced back to the basal levels after MC treatment. In line with the mRNA levels, the protein levels of IL-6 and IL-10 significantly increased in inflammatory cells. MC treatment reversed this increase in protein levels to the baseline as in the control group. Conclusion: We demonstrated in our study the anti-inflammatory effects of MC essential oil on BEAS-2B cells by inhibiting the expression of pro-inflammatory cytokines. To be able suggest MC essential oil as a potential therapeutic agent with a strong anti-inflammatory action, further studies testing several concentrations are warranted.