Dose-Dependent Effects of Clinoptilolite Supplementation on Sperm Quality and Oxidative Stress During Canine Semen Cryopreservation
VETERINARY MEDICINE AND SCIENCE, cilt.12, sa.5, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 5
- Basım Tarihi: 2026
- Doi Numarası: 10.1002/vms3.71128
- Dergi Adı: VETERINARY MEDICINE AND SCIENCE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Hatay Mustafa Kemal Üniversitesi Adresli: Hayır
Özet
Background Cryopreservation induces oxidative stress, leading to reduced semen quality in canine semen. Clinoptilolite (CLP), a natural zeolite, has potential antioxidant properties that may improve post-thaw sperm parameters.Objectives This study aimed to evaluate the dose-dependent effects of CLP supplementation in semen extenders on sperm quality and oxidative stress in dogs.Methods Semen was collected from four adult dogs by manual manipulation, divided into five equal aliquots, and diluted at a ratio of 1:3 (v/v) with a Tris-citrate extender containing 0% (control), 1%, 2%, 3%, or 4% CLP. Samples were cooled to 5 degrees C within 45 min, equilibrated for 2 h in 0.25 mL straws, frozen in liquid nitrogen vapour, and stored in liquid nitrogen. Samples were thawed at 37 degrees C for 30 s. Post-thaw sperm quality was evaluated together with oxidative stress markers, including total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI).Results The 2% CLP group showed the highest post-thaw sperm quality, whereas the lowest performance was observed in the 4% CLP group. Additionally, the 2% CLP group exhibited a more favourable oxidative profile, with higher TAS and lower TOS and OSI values than all other groups.Conclusions CLP supplementation exerted dose-dependent effects on sperm quality and oxidative balance in canine semen cryopreservation. A 2% CLP concentration appears to be the most effective for improving post-thaw sperm quality.