Isolation, characterization, and genomic analysis of vB_Pae_HMKU_23, a lytic bacteriophage against<i> Pseudomonas</i><i> aeruginosa</i> from clinical bovine mastitis

Aslantaş Ö., Nacaroglu M. B., Karaynir A., Turkyilmaz S. S.

ISRAEL JOURNAL OF VETERINARY MEDICINE, cilt.80, sa.3, 2025 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80 Sayı: 3
  • Basım Tarihi: 2025
  • Dergi Adı: ISRAEL JOURNAL OF VETERINARY MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, Jewish Studies Source
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Pseudomonas aeruginosa is a nosocomial pathogen that can cause severe infections in humans and animals. The emergence of multidrug resistance (MDR) among different species of bacteria make phage therapy an attractive alternative for managing infections. In this study, a novel phage, Pseudomonas phage vB_Pae_ HMKU_23, was isolated from urban sewage obtained from a wastewater treatment facility. Phylogenetic analyses revealed that HMKU_23 was a member of Schitoviridae family and Litunavirus genus. The HMKU_23 is a virulent phage as no genes encoding lysogeny, toxins or antibiotic resistance were identified. The therapeutic potential of phage HMKU_23 was investigated and revealed that approximately 66.7% (20/30) of P. aeruginosa strains, isolated from clinical bovine mastitis cases, were lysed by HMKU_23. In contrast, HMKU_23 did not lyse other Gram negative and Gram positive bacteria suggesting its specificity of infection. Collectively, the genetic and biological characteristics of HMKU_23 make it a potential candidate for phage therapy against P. aeruginosa infections.