Chemiresistive CuO sensors for label-free C-reactive protein detection

Bayansal F., Çetinkara H. A.

Journal of Alloys and Compounds, cilt.926, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 926
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.jallcom.2022.166737
  • Dergi Adı: Journal of Alloys and Compounds
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Public Affairs Index, Civil Engineering Abstracts
  • Anahtar Kelimeler: Biosensors, C-reactive protein, Chemiresistor, CuO thin film, Label-free detection, Linear sweep voltammetry
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

In this study, we developed fast and highly sensitive, label-free Ca-doped cupric oxide (CuO) based chemiresistive biosensors to detect C-reactive protein (CRP). The Successive Ionic Layer Adsorption and Reaction (SILAR) method was used to deposit pure and Ca-doped CuO thin films on interdigitated gold electrodes. Film surfaces were functionalized with monoclonal anti-C-reactive protein antibody (anti-CRP) by random physisorption method. Sensor fabrication steps were monitored using the linear sweep voltammetry method. Scanning electron microscopy was used to investigate the surface morphology. Energy-dispersive X-ray spectroscopy was used to determine the elemental composition. The structure of the films was investigated by the powder X-ray diffractometry method. Sensor parameters were determined by the change in sensor resistance over time. The sensors showed a good response to CRP with a linear detection range of 0.1–5.0 µg/ml, which was within the clinically significant range. Detection limits of 49.61 and 48.81 ng/ml were estimated using the standard deviation of the y-intercept and the standard deviation of the regression line, respectively. Dynamic response times of all fabricated sensors were found to be less than 30 s