Protective effects of caffeic acid phenethyl ester, ellagic acid, sulforaphan and curcuma on malathion induced damage in lungs, liver and kidneys in an acute toxicity rat model Effets protecteurs du phenéthyl ester d'acide caféique, de l'acide ellagique, du sulforaphan et du curcumin sur les lésions pulmonaires, hépatiques et rénales induites par le malathion dans un modèle de toxicité aiguë chez le rat

Alp H., Aytekin I., Esen H., Alp A., Buyukbas S., Basarali K., ...More

Revue de Medecine Veterinaire, vol.162, no.7, pp.333-340, 2011 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 162 Issue: 7
  • Publication Date: 2011
  • Journal Name: Revue de Medecine Veterinaire
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.333-340
  • Keywords: γ-glu-tamyltransferase, Acetyl-cholinesterase, Amylase, Antioxidant, Caffeic acid phenethyl ester, Curcuma, Ellagic acid, Kidney, Liver, Lung, Malathion, Rat, Sulforaphan, Toxicity
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

The aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), ellagic acid (EA), sulforaphan (SFN) and curcuma (CUR) against acute malathion (MAL) poisoning in rats. For that, 60 female adult Sprague-Dawley rats were randomly divided into 10 equal groups according to the treatment: whereas one group served as unmedicated control and another was intoxicated with malathion (200 mg/kg, per os) and served as positive control, rats from the other groups were treated with each of the four antioxidants (CAPE: 10 μmol/kg, intraperitoneally, EA: 85 mg/kg, per os, SFN: 0.5 mg/kg, per os and CUR: 1 g/kg, per os) alone or in combination with malathion. One day later, serum AChE (acetylcholinesterase), amylase and GGT (γ-glutamyltransferase) activities were determined and a histopathological evaluation was performed on lungs, kidneys and liver. In MAL-intoxicated rats, the AChE activity was markedly depleted whereas the GGT and amylase activities were significantly increased compared to the unmedicated controls. In parallel, severe and extended inflammatory and degenerative cell lesions were evidenced in liver, kidneys and lungs. By contrast, changes in the serum enzyme activities were greatly attenuated and the organ damage was also markedly reduced but not completely abrogated when an antioxidant cotreatment has been instituted. In addition, CUR appeared as the more efficient for hindering biochemical and histopathological alterations induced by malathion. These results show the protective effects of CAPE, EA, SFN and CUR on acute malathion poisoning in rats.